Timely culture for mycobacteria which utilizes a microcolony method
نویسندگان
چکیده
منابع مشابه
Early Detection of Mycobacteria Using a Novel Hydrogel Culture Method
BACKGROUND Early laboratory detection of Mycobacterium tuberculosis is crucial for controlling tuberculosis. We developed a hydrogel mycobacterial culture method that retains the advantages of both solid and liquid methods in terms of speed, cost, and efficiency. METHODS Mycobacterium bovis bacillus Calmette-Guérin (BCG) suspensions and 200 acid-fast bacilli (AFB)-positive clinical specimens ...
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digestion and decontamination was done by the NaOH (Sodium Hydroxide) -NALC (N-acetyl-L-cysteine) method, followed by centrifugation for 20 minutes at 3000 revolutions/minute. 0.5 ml of each concentrate was added into the 3 media. MGIT vials were monitored by the MGIT 960 system while the Bio FM and LJ media were examined upto 42 days and 56 days respectively for mycobacterial growth. Isolates ...
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We confirmed that chlorhexidine decontamination yielded more nontuberculous mycobacteria than did the N-acetyl-l-cysteine-NaOH-oxalic acid procedure from respiratory samples of cystic fibrosis patients on solid cultures. However, this improved recovery is mostly balanced if the latter is combined with liquid culture. Furthermore, none of the 145 cough swabs, used to sample young children, cultu...
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The identification of mycobacteria is essential because tuberculosis (TB) and mycobacteriosis are clinically indistinguishable and require different therapeutic regimens. The traditional phenotypic method is time consuming and may last up to 60 days. Indeed, rapid, affordable, specific and easy-to-perform identification methods are needed. We have previously described a polymerase chain reactio...
متن کاملCulture of specimens other than sputum for Mycobacteria.
A comparison has been made of Lowenstein-Jensen medium, with or without added pyruvate, 7H11 oleic acid-albumin agar slopes made selective by the addition of antibiotics, and selective Kirchner medium in the isolation of tubercle bacilli from specimens other than sputum. A combination of a standard mild preliminary treatment with sulphuric acid and selective medium reduced the contamination rat...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1993
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.31.8.2178-2184.1993